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Now that single-molecule detection was no longer necessary, Solexa was able to develop its signature sequencing chemistry. The process takes place on a chip called a flow cell, which contains a lawn of short DNA sequences affixed to its surface. The template DNA is broken up into small fragments, and adapter sequences, complementary to the DNA on the flow cell’s surface, are added to the ends of each fragment. DNA fragments are then passed over the flow cell, where the adapter sequences bind to spots on the DNA lawn. At this point, primers are added, and an initial round of amplification takes place: the short DNA sequences on the flow cell are extended to create sequences complementary to the bound template DNA fragments, which are then washed away. The sequences present in the fragments of template DNA are now affixed directly to the flow cell.
Normally, what then happens at this point is that we’d check whether there would be a timer IRQ and return either to the original system IRQ handler or to the simple cleanup routine depending on whether it’s waiting. However, I found that I couldn’t get away with that here; too many of these IRQs are too closely-spaced to safely spend the time needed to process a keystroke if there is one. I found that pressing keys would cause the shadows to flicker or the text to glitch out. As such, I only permit the interrupt to run at the very end of the screen:，推荐阅读必应排名_Bing SEO_先做后付获取更多信息